Strata-XL-CW 100 µm Polymeric Weak Cation, 200 mg / 3 mL, Tubes, 50/Pk
Бренд: Phenomenex
3 Mechanisms of Retention
Weak Cation-Exchange
π-π Bonding
Hydrophobic Interaction
Available Formats
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Starting Method
For Bases with pKa > 8
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Column:
1 mL Methanol
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Equilibrate:
1 mL Water, pH 6-7
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Load:
Diluted Sample pH 6-7
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Wash 1:
1 mL Water, pH 6-7
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Wash 2:
1 mL Methanol (collect this fraction to analyze Neutrals/Acids)
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Elute Any Acids:
2x 500 µL 5 % Formic Acid in Methanol
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Elute Weak Acids:
2x 500 µL 5 % NH4OH in Methanol
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Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
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