Waters XBridge 125Å SEC Column, 2.5 µm, 4.6 x 300 mm (BEH-DIOL based) with MaxPeak Premier Technology was developed for scientists working on biotherapeutic peptides (e.g., GLP receptor agonist and insulin-like)and small proteins ,from approx 1K — 80K Dalton, who seek higher resolution of size variants, more reproducible HMWPs quantitation with reduced run times and solvent cost savings. Waters expertise in SEC separations coupled with use of performance enhancing MaxPeak High Performance Surface (HPS) Technologies and innovative manufacturing processes address existing scientific challenges that include minimizing non-desired secondary ionic interactions between the biomolecules and the surfaces of the SEC column hardware.
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Chemistry
SEC
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Separation Mode
SEC
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Particle Substrate
Hybrid
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pH Range Min
2.5 pH
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pH Range Max
8 pH
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Temperature Limits
60 C
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Maximum Pressure
7500 psi (517 Bar)
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Endcapped
No
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Bonding Technology
Diol (OH)
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Silanol Activity
Low
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Molecular Weight Range Min
1000
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Molecular Weight Range Max
80000
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Particle Shape
Spherical
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Particle Size
2.5 µm
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Endfitting Type
Parker-style
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Pore Size
125 Å
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QC Tested
Peptide and Protein
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Format
Premier Column
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Packing Solvent
10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl
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Surface Area
220
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System
HPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L20, L33, L67
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Inner Diameter
4.6 mm
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Length
300 mm
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Carbon Load
12 %
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eCord
No
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UNSPSC
41115711
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Application
Protein
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Brand
XBridge
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Product Type
Columns
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Units per Package
1 pk