The XBridge Premier Protein BEH C4 300 Å 2.5 µm, 4.6 x 100 mm columns are QC tested with the MassPREP Protein Mix standard (p/n: 186004900) and are optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.
Note: The same column is also available with the MassPREP Protein Mix Standard at no additional cost (p/n: 176005114)
Suggested use: Low-level intact protein and subunit analysis, phosphorylated proteins. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software.
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Chemistry
C4
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Separation Mode
Reversed Phase
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Particle Substrate
Hybrid
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pH Range Min
2 pH
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pH Range Max
10 pH
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Maximum Pressure
10000 psi (690 Bar)
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Endcapped
No
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Silanol Activity
Low
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Molecular Weight Range Max
150000
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Particle Shape
Spherical
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Particle Size
2.5 µm
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Endfitting Type
Parker-style
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Pore Size
300 Å
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QC Tested
Protein
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Format
Premier Column
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Surface Area
90
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System
UHPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L26
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Inner Diameter
4.6 mm
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Length
100 mm
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UNSPSC
41115711
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Application
Protein
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Brand
XBridge
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Product Type
Columns
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Units per Package
1 pk
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eConnect
Yes