XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 7.8 mm X 300 mm Column, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 1,000 – 80,000 Daltons. Each batch is specifically QC tested using Waters BEH125 SEC Protein Standard (p/n: 186006519) to help ensure consistent performance for challenging size-based protein separations.
Note: This XBridge Protein BEH SEC, 125Å Column is also available with a vial of BEH125 Protein Standard at no additional charge (p/n: 176004324)
Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis
-
Chemistry
SEC
-
Separation Mode
Gel Filtration (aqueous)
-
Particle Substrate
Hybrid
-
pH Range Min
2.5 pH
-
pH Range Max
8 pH
-
Temperature Limits
60 C
-
Maximum Pressure
7500 psi (517 Bar)
-
Endcapped
No
-
Bonding Technology
Diol (OH)
-
Silanol Activity
Low
-
Molecular Weight Range Min
1000
-
Molecular Weight Range Max
80000
-
Particle Size
2.5 µm
-
Endfitting Type
Parker-style
-
Pore Size
125 Å
-
QC Tested
Protein
-
Format
Online Column
-
Packing Solvent
20% methanol in water
-
Surface Area
220
-
System
UHPLC, UPLC, HPLC
-
Particle Technology
BEH
-
Technique
LC
-
USP Classification
L20, L33, L59
-
Inner Diameter
7.8 mm
-
Length
300 mm
-
Carbon Load
12 %
-
eCord
No
-
UNSPSC
41115711
-
Application
Protein
-
Brand
XBridge
-
Product Type
Columns
-
Units per Package
1 pk