XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 300 mm Columns, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 to 450,000 Daltons. Each batch is specifically QC tested using Waters BEH200 SEC Protein Standard (p/n: 186006518) to help ensure consistent performance for challenging size-based protein separations.
Note: This item is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004336).
Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis
If you are developing a new method, please consider our newer technology XBridge Premier Protein 250Å SEC and ACQUITY Premier Protein 250Å SEC Columns for superior recovery, separation, and resolution.
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Chemistry
SEC
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Separation Mode
Gel Filtration (aqueous)
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Particle Substrate
Hybrid
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pH Range Min
2.5 pH
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pH Range Max
8 pH
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Temperature Limits
60 C
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Maximum Pressure
6,500 psi (448 Bar)
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Endcapped
No
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Bonding Technology
Diol (OH)
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Silanol Activity
Low
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Molecular Weight Range Min
10000
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Molecular Weight Range Max
450000
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Particle Size
2.5 µm
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Endfitting Type
Parker-style
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Pore Size
200 Å
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QC Tested
Protein
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Format
Online Column
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Packing Solvent
20% methanol in water
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Surface Area
220
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System
UHPLC, UPLC, HPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L20, L33, L59
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Inner Diameter
4.6 mm
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Length
300 mm
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Carbon Load
12 %
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eCord
No
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UNSPSC
41115711
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Application
Protein
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Brand
XBridge
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Product Type
Columns
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Units per Package
1 pk